The discipline "Protein Engineering" was developed to the students with
the basic knowledge of the molecular biology and protein engineering
approaches for the production of complex biomolecules and their
investigation by methods of molecular and structural biology.
The discipline forms in students’ complex competencies in the field of
nanobiotechnology, including comprehension of applicability of protein
engineering methods for the studies of biological objects, such as
macromolecules and also for processing, analysis and synthesis of
information about modern achievements in molecular and structural
biology.
Study the discipline "Protein Engineering" provides the students the
knowledge about modern methods of biomacromolecules production in
various expression systems, methods of isolation, purification and
refolding of globular and membrane proteins.
The discipline covers issues related to the application of methods of rational
design and directed evolution for protein studies. Also issues regarding
studying of the functional activity of proteins in various systems, including in
vivo approaches in cells and whole organisms, are considered in details. The
course highlights the advantages of the integrated interdisciplinary approach
in solving problems of molecular and structural biology.
- Преподаватель: ShulepkoMikhail