Course info

Aim of the course

The course of "Raman spectroscopy" is the theorethical course that introduces Raman spectroscopy method to master students specializing in "Nanobiotechnology". Raman spectroscopy is a modern powerful approach for studying biological systems and nanostructured materials. The course aims to provide students with theoretical information about the physical foundations of Raman spectroscopy and its biological application. The course also includes basic knowledge about physical mechanisms of spectroscopy and reviews modern spectroscopic methods, other than Raman spectroscopy.

Annotation

The teaching course deals with theory of Raman scattering phenomenon, including the classical and quantum mechanical approach to explain Raman effect. The Rayleigh, Stokes and anti-Stokes scattering is considered. The vibrational properties of molecules are analyzed, the connection of chemical structure and symmetry of molecules to their ability to produce Raman or IR lines in spectrum are reviewed.

The course considers some practical information about Raman spectroscopy equipment and the spectra acquisition and processing software. The species of Raman spectroscopy such as Resonance Raman spectroscopy and Surface Enhanced Raman spectroscopy as well as the mechanisms underlying these processes are presented. The course gives general information about more advanced Raman spectroscopy approaches such as Coherent anti-Stokes Raman spectroscopy (CARS) and Tip-Enhanced Raman spectroscopy (TERS) and other modern modifications of the method. The central part of the course dedicated to practical application of Raman spectroscopy in biology, the information of the Raman spectra of the main components of living cells is presented, the approaches of living cells studying are mentioned.

The course combines presentation of theoretical material and discussions on the corresponding topics. During the course the students make hometasks in the form of tests on the Moodle platform and as a practical work with Renishaw software.

1. Title of the discipline topics

Topic 1. Introduction. Properties of light (4h)

Topic 2. Introduction. Structure of matter (4h)

Topic 3. Introduction. Light interaction with matter (2h)

Topic 4. Introduction. Spectroscopy (2h)

Topic 5. Raman spectroscopy theory (2h)

Topic 6. Vibrations. Symmetry and Group Theory (4h)

Topic 7. Polarizability. Raman spectrum (4h)

Topic 8. Raman spectrometer construction. Spectra processing (4h)

Topic 9. Spectra of biological molecules. Resonance Raman scattering (4h)

Topic 10. SERS and More Advanced Raman Scattering Techniques (4h)

Topic 11. Application of Raman spectroscopy (2h)

Duration of academic discipline (course)

36 classroom hours (seminars)

9 weeks

Reporting form

Exam

Maximum Points/Percentage

100 points (100%)

The structure of the final assessment (how many percent / points of the final assessment each of the types of student's work takes)

Course attendance is mandatory. Completing the home tasks can bring a pass to the final exam. Active participation in seminar can deliver 10 points. The presentation on the seminar can bring 15 points. The intermediary test gives 25 poins. The course is terminated with the exam, exam tasks give 50 points. Total score of 86-100 points corresponds to final excellent grade on the course, 73-85 – Good, 60-72 – Satisfactory, <60 – Unsatisfactory

How many classes can be missed without a good reason?

0

List of recommended literature

Basic educational and methodological literature

1. Banwell, Fundamentals of molecular spectroscopy 3d edition, 1983

2. E.Smith, G.Dent, Modern RamanSpectroscopy– A Practical Approach, 2005

3. J. Toporski, Th.Dieing, O.Hollricher Confocal Raman Microscopy, 2d Edition, 2018

Supplementary educational and methodological literature

1. Gordon G. Hammes Spectroscopy for the Biological Sciences, 2005

Resources of information in the telecommunications network "Internet"

1. Molecular symmetry From Wikipedia, the free encyclopedia https://en.wikipedia.org/wiki/Molecular_symmetry

2. List of character tables for chemically important 3D point groups From Wikipedia, the free encyclopedia https://en.wikipedia.org/wiki/List_of_character_tables_for_chemically_important_3D_point_groups

3. Hybridization Theory https://www.youtube.com/watch?v=8VBs_xf7yLs

4. Hybridization Theory https://www.youtube.com/watch?v=wPw_LCmyjnI

5. Group theory https://www.youtube.com/watch?v=mH0oCDa74tE

6. Symmetry Introduction https://www.youtube.com/watch?v=PzLD41m68jk

7. Symmetry: IR and Raman Spectroscopy https://www.youtube.com/watch?v=1Q45PpodjJY

8. Google Scholar search engine for tracking scientific advances in a subject of study based on journal publications

7. The Internet Encyclopedia of Philosophy (IEP) http://www.iep.utm.edu/